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17504-044B-27® 添加劑是無血清添加劑

發布日期: 2019-04-09
瀏覽人氣: 528

杭州仟諾生物科技有限公司17504-044B-27® 添加劑是無血清添加劑 N2等常用試劑大量現貨備貨,到貨

 

B-27® 添加劑是無血清添加劑,用于海馬神經元和其他中樞神經系統(CNS)神經元的生長和保持其短期或長期活性。B-27® 添加劑是50X 的液體,可以與Neurobasal® 培養基或Neurobasal®-A 培養基組合使用,用于神經細胞培養而不需要再添加飼養層細胞。

B-27® 添加劑適和應用于大多數神經細胞培養。對于特別的應用,我們提供 其他配方的B-27® 添加劑.

質量控制檢測 
B-27® 添加劑 進行了18天妊娠的原代大鼠(Sprague Dawley) 的胚胎海馬神經元生長檢測。 通過了細菌和真菌的檢測. B-27® 也做了1X 濃度的內毒素水平測試。 

僅供研究使用。除非另有說明,不得用于任何動物或人類治療或診斷。

詳細說明

B-27® 添加劑是無血清添加劑

常用規格

Cell Type:

Neuronal Cells

Product Size:

10 ml

Classification:

Serum-Free

Other Additives:

Vitamin A,

Antioxidant Cocktail,

Insulin

Purity or Quality Grade:

Cell Culture Grade

Green Features:

Sustainable packaging

Shipping Condition:

Dry Ice

Regulatory Statement:

For Research Use Only。 Not for use in diagnostic procedures。

引文和參考文獻 (17)

  • D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者

Congar P; Bergevin A; Trudeau LE

雜志

J Neurophysiol (2002) 87:1046-1102

產品用途

Using a culture system in which isolated DAergic neurons establish self-innervating synapses ("autapses") that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents (EPSCs).

ID:

PN64425

  • Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice。

作者

Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;

雜志

Neuron (2002) 33:789-804

產品用途

Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.

ID:

PN47418

  • Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells。

作者

Kabos Peter; Kabosova Andrea; Neuman Toomas;

雜志

J Biol Chem (2002) 277:8763-8769

產品用途

Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized (0.02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement (Invitrogen). Cells were washed with PBS (Invitrogen)

ID:

PN63471

  • Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death。

作者

Lesuisse Christian; Martin Lee J;

雜志

J Neurobiol (2002) 51:9-23

產品用途

A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.

ID:

PN60461

  • An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者

Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;

雜志

Brain Res Brain Res Protoc (2002) 9:93-104

產品用途

Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties

ID:

PN62028

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